Accordion is a Tiki which appears in the boss level of World 7, Colonel Pluck. Accordion hypnotizes Pluck into making the Stompybot 3000 to squash bananas which power up the Tiki minions. When Donkey and/or Diddy Kong appear, they are forced to battle Pluck. When he is defeated, Accordion rises up and Donkey Kong sends him flying away. Later, he is seen with the rest of the hypnotic tikis by becoming part of one of Tiki Tong's hands.

Accordion's tune is Kalimba's base tune with an accordion playing in the background in the same fashion of Kalimba's tune.

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